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Special Staining
The
picture " 
" can link to it's
corresponding histological picture.
The picture " 
" can link to it's
corresponding histological drawing.
 Azan stain:
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»» Nuclei,
osteocytes, neuroglia - bright red
»» Cytoplasm - pink to blue
»» Collagen and reticulum - blue
»» Muscle and RBC - red to yellow |
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Bielschowski's method:
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»» Intracellular neurofibrils - black
»» Axons - black |
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Mallory's aniline blue collagen stain:
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»» Nuclei - red
»» Collagenous fibrils - blue
»» Ground substance, cartilage,
mucin, amyloid
- varying shades of blue
»» RBC, myelin - yellow
»» Elastic fibrils - pale pink, pale yellow, or unstained |
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PAS stain:
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»» Carbohydrates - deep red
»» PAS positive - mucin, basement
membrane, brush border, glycogen |
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Osmium tetroxide for lipids
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»» Fat - black
»» Background - yellow to brown |
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Silver and gold methods for nerve tissue:
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»» Neuron process - black, brown or golden |
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Silver stain for reticular fibrils
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»» Reticular fibers - black |
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Weigert's resorcin-fuchsin stain for elastic fibers:
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»» Elastic fibers - blue-black to black
»» Nuclei - blue to black
»» Collagen - pink-red
»» Other tissue elements - yellow |
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